Cytomorphologic Characteristics of Next-Generation Sequencing-Positive Bile Duct Brushing Specimens

Course overview

LEARNING OBJECTIVES Recognize and analyze the challenges associated with interpreting cytologic specimens of Bile Duct Brushings (BDBs), including the complexity of overlapping morphologic features between non-neoplastic, low-grade neoplastic, and malignant bile duct strictures. Establish a correlation between specific cytomorphologic characteristics observed in BDB samples and the presence of genetic mutations associated with high-risk strictures, using Next-Generation Sequencing (NGS) as a diagnostic tool. Evaluate the potential impact and clinical implications of incorporating NGS into BDB cytology algorithms, considering the improved sensitivity and diagnostic accuracy in identifying high-risk and malignant strictures.