Virtual Program: A Xylene Free Veterinary Diagnostic Laboratory: Creating a Safer Working Environment

Course overview

Xylene is a Class D aromatic hydrocarbon used daily in the histology laboratory for tissue processing, slide staining, processor cleaning and the removal of coverslips. Class D chemicals are known to cause temporary or permanent adverse health effects. Due to its toxicity, short-term inhalation exposure may lead to eye, nose, and throat irritation, and gastrointestinal and neurologic effects. Long-term exposure may result in headaches, dizziness, fatigue, tremors, and incoordination. Respiratory, cardiovascular, and kidney issues may also arise. Reducing the exposure of personnel to xylene in the laboratory should be achieved. Xylene alternatives should be investigated and tested through a slide validation process. Alternatives range from unconventional methods using liquid dishwashing soap and cedarwood oil to commercially available alternatives such as isopropanol or SlideBrite. Each laboratory has different needs and requirements for tissue processing and staining. Each laboratory must validate their chosen xylene alternative to validate the method works with their processor and stainer.