PROTEIN ELECTROPHORESIS

Course overview

Interpretation of protein electrophoresis patterns from serum, urine, and cerebrospinal fluid Learning Objectives 1. Describe the methods of agarose gel electrophoresis, immunofixation, and densitometry. 2. Name the clinical conditions most commonly associated with monoclonal gammopathy, and some key clinical features of each condition. 3. View an agarose gel, an immunofixation, and a densitometry and determine if a monoclonal gammopathy is present. If it is present, the user will be able to identify the immunoglobulin type. 4. View an agarose gel of serum, urine, or cerebrospinal fluid and identify the electrophoresis pattern (e.g., inflammation, liver disease, hemolysis, alpha-1 antitrypsin deficiency, tubular proteinuria, CSF oligoclonal banding, etc).