FLOW CYTOMETRY BLOOD CELL IDENTIFICATION

Course overview

FLOW CYTOMETRY IS A STATE-OF-THE-ART TECHNIQUE BASED ON THE PRINCIPLES OF LIGHT SCATTERING AND FLUORESCENCE EMISSION WHEN CELLS ARE INTERROGATED BY A LASER BEAM. THIS TECHNIQUE ALLOWS FOR BOTH QUALITATIVE AND QUANTITATIVE ASSESSMENT OF CELLS IN SUSPENSION BASED ON THEIR PHYSICAL PROPERTIES AND THE EXPRESSION OF IMMUNOPHENOTYPIC MARKERS. THIS RAPID AND COST-EFFECTIVE METHOD IS NOW WIDELY AVAILABLE, PARTICULARLY RENOWNED FOR ITS ROLE IN THE IMMUNOPHENOTYPING OF HEMATOLYMPHOID NEOPLASMS. APART FROM THIS WELL-KNOWN UTILITY, THE POTENTIAL ROLE OF FLOW CYTOMETRY IS BEING EXPLORED FOR MANY OTHER DISEASE CONDITIONS. UNDERSTANDING THE BASICS OF FLOW CYTOMETRY IS NECESSARY TO PROVIDE OPTIMAL PATIENT CARE AND DIAGNOSTICS. ALSO, THIS KNOWLEDGE EMPOWERS THE RESEARCHER TO EXPLORE THE POTENTIAL APPLICATIONS OF FLOW CYTOMETRY. THIS COURSE DISCUSSION FOCUSES ON UNDERSTANDING THE PRINCIPLE OF THE TECHNIQUE, IDENTIFYING THE CELL POPULATIONS ON FLOW CYTOMETRY, AND THE CURRENT USES OF FLOW CYTOMETRY.