MEDUSA: Maintaining Entire DNA Duplexes for Utmost Sequencing Accuracy
Tracking # 20-1396540
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Learn moreCourse overview
This webinar will introduce MEDUSA (Maintaining Entire DNA Duplexes for Utmost Sequencing Accuracy), a novel, streamlined library preparation method designed to mitigate duplex sequencing errors without sacrificing genome coverage. We will explore the mechanics of how trace dNTP carryover from end repair drives unintended strand resynthesis during dA-tailing, and explain how integrating an apyrase-mediated enzymatic step effectively blocks this resynthesis. Participants will discover how MEDUSA performs across challenging clinical specimens--including formalin-fixed paraffin-embedded tumor DNA and cell-free DNA--achieving ultra-low residual variant frequencies while maintaining about 100% genome coverage and excellent molecular recovery. Attendees will walk away with actionable insights on how to balance absolute sequencing accuracy with complete genomic breadth, making this session essential for anyone looking to optimize liquid biopsies, rare-variant assays, and clinical oncology workflows.
Subject areas
This course counts toward the state boards and subject areas below.
American Board of Clinical Chemistry
Clinical Chemistry Diplomate
Molecular Diagnostics Diplomate
Toxicological Chemistry Diplomate
Florida Board of Clinical Laboratory Personnel
Clinical Laboratory Director
Clinical Laboratory Supervisor
Clinical Laboratory Technician
Clinical Laboratory Technologist
Public Health Director
Public Health Supervisor
Public Health Technologist
Disclosure statements
The Association for Diagnostics & Laboratory Medicine is dedicated to ensuring balance, independence, objectivity, and scientific rigor in all educational activities. All participating planning committee members and faculty are required to disclose to the program audience any financial relationships related to the subject matter of this program. Disclosure information is reviewed in advance in order to manage and resolve any possible conflicts of interest. The intent of this disclosure is to provide participants with information on which they can make their own judgments.
The following faculty reported no financial relationships:
- Linnea M. Baudhuin, PhD, DABMGG, FACMG
- Kan Xiong, PhD