Bringing Flow Cytometry to Life: Applications in Cellular Therapies

Course overview

Flow cytometry plays an integral role in the modern laboratory. This rapid, high throughput, multiparameter assay is used to phenotype, characterize, and assess cell function by measuring marker expression on the cell surface and inside cells. It is an important tool that helps to ensure quality, purity, safety and/or potency of products used in cellular therapies and regenerative medicine. Learning how flow cytometry is applied in practice is essential to maximizing its power to collect and interpret data for clinical and research applications. AABBs course, Bringing Flow Cytometry to Life: Applications in Cellular Therapies: Teaches the basics of flow cytometer operation Through the use of actual examples, students will experience how flow cytometry can be applied in cellular therapies (CT) providing a real-world experience and bringing flow cytometry to life. The course is divided into three sections that highlight important aspects of flow cytometry practice and application for CT. Students will test and apply knowledge in a series of exercises. A glossary and list of supporting reference material is also included. Section 1: Foundational Concepts in Flow Cytometry covers the essential components of a flow cytometer and basic principles in setting up the machine, cell surface staining and data analysis. Section 2: Cell Therapy Applications Using Flow Cytometry in the Cell Processing Laboratory - presents real world case studies demonstrating how flow cytometry concepts are applied in practice. Students learn through a series of practice exercises using examples of cell products widely processed in CT laboratories. Section 3: Review of Concepts: Applying What You Have Learned interactive exercises for further practice of the concepts covered in the program.