Multiplexing on Animal and Xenograft Tissue (OLC)

Course overview

Every doctor and investigator wants to know three things: can it be done, how long will it take, and how much will it cost? Multiplexing is an immunohistochemical technique that allows for the visualization of multiple antigens on the same slide. The ability to visualize multiple antigens through fluorescence or chromogenic staining saves time, money, tissue, and reagents. In order to do this successfully, it is necessary to know how to properly develop a protocol. Issues such as background staining, non-specific staining, species to species reactivity, and lack of protocol development tactics can hinder the efficient use of multiplexing. This workshop will walk histology professionals through antibody and reagent selection for all tissue types with consideration of species to species reactivity. It will also address concerns of proper control material selection, antigen retrieval, chromogen usage and staining order. Developing a successful multiplexing protocol can be a daunting task, but with the tools from this workshop it is a task anyone can accomplish