PCR Fundamentals

Course overview

The polymerase chain reaction is arguably one of the most common and useful molecular experimental techniques employed in diagnostic laboratories today. In the laboratory, the uses for PCR include disease and infection diagnosis, mutation detection, pathogen or pathogenic response detection, and template amplification for downstream applications such as sequencing. The goal of this course is to give the laboratory professional an overview and review of polymerase chain reaction and its uses in the laboratory as well as to provide the technologist with helpful tools for troubleshooting when reactions do not proceed as planned.