IHC Troubleshooting

Course overview

While using the right antibody to target the antigen and amplify the signal is obviously the most crucial component in reliable immunohistochemical (IHC) visualization, all of the steps in the IHC process are important for generating great images. Sub-optimal IHC staining is common, but can be corrected by adjusting some of the variables in the workflow steps, such as sample preparation, antigen retrieval and incubation times, to name a few. Whether you are new to IHC or an experienced researcher wanting to confirm your method, consider these five critical steps to help ensure that your images are publication ready the first time.