Basic IHC: Understanding the Process to Improve Your Results

Course overview

Immunohistochemical staining is now so commonly performed by instruments or robots that the actual staining process is lost in the barcodes and software, bottles, and vials. This workshop dives back down to the basics of what is really happening on the slide: from tissue preparation to the sequential application of primary antibody, linking antibodies, enzyme complex, chromogen and counterstain. We will discuss the effects that pre-analytical events such as fixation, processing and microtomy have on the staining process as well as the result